Microscopes

TP – our cleared tissue digital scanned light sheet microscope. The first inertia-free, adaptive, and autofocusing light-sheet. Detailed in our Nature Communications paper. TP is currently undergoing a major upgrade to add adaptive optics with indirect wavefront sensing into the detection pathway and is not running.

Nila – our traveling tissue light sheet microscope. Nila is a cost-effective instrument using multiple ASI LS-50 stages, an Olympus macroscope objective, Optotune electrotunable lens, and a 20 megapixel industrial FLIR CMOS camera to provide easy to use tiled light sheet imageswith uniform axial resolution in cleared tissue. A lot of what we learned about autofocusing and autoalignment from TP is now packaged into Nila. We are working on getting a GitHub up and running with a parts list, build instructions, Micromanager 2.0 gamma code, and image fusion via deconvolution code.

Our traveling light sheet at the 2019 ATS meeting.

Sid – our multiple color incoherent SIM with a Bioptech FCS2 chamber and objective heater for live-cell super-resolutionimaging. Sid uses a modified version of the MAP-SIM framework for DMD calibration and 3D SIM reconstruction. Sid has eight LED channels, a programmable DMD, multiple objectives, fully automated XYZ stage, and two cameras. Sid is detailed in our preprint on a spatially localized drug response in primary endothelial cells.

Overhead view of the incoherent SIM and results from an Argolight calibration slide.

Max – our 3D dSTORM instrument. Max is based on the ZOLA-3D architecture and has a Pumpy fluidics unit attached to it for iterative labeling and localization microscopy. We use our 3D printed version of Pumpy, which was designed by Leonardo Saunders in the lab.

Our TIRF with PSF manipulation for localization microscopy (left) and 3D printed pumpy setup for iterative labeling (right).