Microscopes

macroSPIM– our cleared tissue digital scanned light sheet microscope. The first inertia-free, adaptive, and autofocusing light-sheet. Detailed in our Nature Communications paper. The tiling macroSPIM is currently undergoing a major upgrade with new cameras, DAQs, and on-line fusion for uniform resolution and image quality. We hope to have the Github and preprint public soon!

fastDMDSIM – we demonstrated the first multicolor DMD-based SIM, based on a new model of light propagation through the system. We also created the first full Python package for simulating, creating SIM patterns, running the microscope, and analyzing SIM data. Equipped with a Bioptech FCS2 chamber and objective heater for live-cell super-resolution imaging.
Github
Preprint

OPM – our oblique plane microscope using a bespoke solid immersion objective. This instrument also has our fluidics system for MERFISH. This system has a larger scan area than the stage scanning Lattice Light Sheet, with a better lateral resolution and near or equivalent axial resolution.
Github
Publication