Microscopes

All of our microscopes are built around open-source software (either Micro-Manager or Python), Arduino or Teensy microcontrollers, and do it yourself optical plans.

As we have time, we will update with links to GitHub for each individual microscope, fluid handling robots, and protocols.

TP – our cleared tissue digital scanned light sheet microscope. The first inertia-free, adaptive, and autofocusing light-sheet. Detailed in our Nature Communications paper. TP has seven excitation lines, multiple cameras, multiple Optotune electrotunable lenses, index-matched detection objectives, automated fluid handling for seqFISH, and automated tiling of large-samples. TP is controlled via a Teensy microcontroller, Python control software, and a modified OpenStage controller.

Cooper- our modular OpenSPIM variant. Cooper has three lasers, two cameras, three swappable samples chambers for either: high-NA excitation with 16X or 40X imaging of development or low-NA excitation with 10X index-matched imaging of cleared tissue, and an adaptive extended field of view light-sheet. Cooper is controlled via an Arduino microcontroller and the OpenSPIM framework. Currently installed and operated as part of the University of Colorado Anschutz Medical Campus Advanced Light Microscopy Core. A publication is in preparation.

Sid – our multiple color LED-SIM with automated fluid handling for sequential multiplexing or live-cell imaging. Sid has four LED channels, a programmable DMD, multiple objectives, fully automated XYZ stage, and multiple cameras. Sid is controlled via a Teensy and MicroManager 2.0. Sid is detailed in an upcoming publication characterizing endothelial progenitor cell heterogeneity.