TP – our cleared tissue digital scanned light sheet microscope. The first inertia-free, adaptive, and autofocusing light-sheet. Detailed in our Nature Communications paper. TP is currently undergoing a major upgrade to add adaptive optics with indirect wavefront sensing into the detection pathway and is not running.
Nila – our traveling tissue light sheet microscope. Nila is a cost-effective instrument using multiple ASI LS-50 stages, an Olympus macroscope objective, Optotune electrotunable lens, and a 20 megapixel industrial FLIR CMOS camera to provide easy to use tiled light sheet imageswith uniform axial resolution in cleared tissue. A lot of what we learned about autofocusing and autoalignment from TP is now packaged into Nila. We are working on getting a GitHub up and running with a parts list, build instructions, Micromanager 2.0 gamma code, and image fusion via deconvolution code.
Sid – our multiple color incoherent SIM with a Bioptech FCS2 chamber and objective heater for live-cell super-resolutionimaging. Sid uses a modified version of the MAP-SIM framework for DMD calibration and 3D SIM reconstruction. Sid has eight LED channels, a programmable DMD, multiple objectives, fully automated XYZ stage, and two cameras. Sid is detailed in our preprint on a spatially localized drug response in primary endothelial cells.
Max – our 3D dSTORM instrument. Max is based on the ZOLA-3D architecture and has a Pumpy fluidics unit attached to it for iterative labeling and localization microscopy. We use our 3D printed version of Pumpy, which was designed by Leonardo Saunders in the lab.