An NIH funded T32 postdoctoral fellow position (USA citizenship or USA permanent residency required) is available in the Shepherd laboratory in the Department of Pharmacology and Pediatric Heart Lung Center at the University of Colorado School of Medicine in Aurora CO. The focus of the research program on understanding how endothelial progenitor cell gene expression and differentiation is regulated in developing mammalian organs. We are particularly focused on vascular formation in the brain and lung in murine models of disrupted fetal development. The applicant will be responsible for the design, implementation, and application of multiplexed RNA imaging assays using optical tissue clearing, light sheet microscopy, and machine vision to map spatial transcriptome of endothelial progenitor cells. The ideal applicant will have a biophysics, developmental biology, or molecular and cellular biology background with an interest in the spatial regulation of stochastic genetic regulation.
Please look at the requirements below. If interested, please contact Dr. Shepherd at email@example.com with a cover letter and CV.
- Must be able to sustain prolonged periods of sitting and/or standing in the laboratory environment.
- Must be willing to work with animal models.
- Must be willing to work in a standard research environment that may contain lasers, electronics, fumes, chemicals, liquid nitrogen, sharp objects, hazardous materials, tissue, and body fluids.
- Must be willing to work on multi-disciplinary projects with a biological emphasis.
- Funding for this position requires that the selected individual hold US citizenship or a US permanent residence.
- Graduation from an accredited college or university with a recent PhD in Molecular or Cellular Biology, Biochemistry, Developmental Biology, Biophysics or a related field.
- Minimum of 3 years experience in physical sciences.
- Excellent communication skills in English
- Proven ability to work independently within a research lab
- Demonstrated ability to work with cell and tissue cultures
- Demonstrated ability to perform immunofluorescence labeling
- Demonstrated ability to perform in situ labeling
Desired or preferred:
- Experience with animal models
- Experience with ex vivo lung explants
- Experience with light sheet fluorescence microscopy
- Experience with quantitative image analysis
- Experience with basic statistical analysis
- Applicants should be highly-motivated and accomplished, interested in creative approaches, and have (or anticipate having) a recent PhD in Molecular or Cellular Biology, Biochemistry, Developmental Biology, Biophysics or a related field.